They have the same functions as the contractile vacuole of freshwater Protozoa.
In Polia the connective tissue enclosed in the external muscular layer is eminently vacuolar - all the intermediate stages between such cells in which the vacuole predominates and the nucleus is peripheral and those in which the granular protoplasm still entirely fills them being moreover present.
The naked cells which have been alluded to live in water, and call therefore for no differentiation in connection with this necessity; but those which are surrounded by a cell-wall always develop within themselves a vacuole or cavity which occupies the greater part of their interior, and the hydrostatic pressure of whose contents keeps tha protoplasm in contact with the membrane, setting up a condition of turgidity.
In Gnetum Gnemon, as described by Lotsy, a mature embryo-sac contains in the upper part a large central vacuole and a peripheral layer of protoplasm, including several nuclei, which take the place of the archegonia of Ephedra; the lower part of the embryo-sac, separated from the upper by a constriction, is full of parenchyma.
Eventually the protoplasm usually forms only a lining to the cell wall, and a large vacuole filled with cell sap occupies the centre.
In the yeast cell the nucleus is represented by a homogenous granule, probably of a nucleolar nature, surrounded and perhaps to some extent impregnated by chromatin and closely connected with a vacuole which often has chromatin at its periphery, and contains one or more volutin granules which appear to consist of nucleic acid in combination with an unknown base.
Young cells ar full of cytoplasm, old cells generally contain a large vacuole or vacuoles, containing cell-sap, and with only a thin, almost invisible layer of cytoplasm on their walls.